研究人员为解决 Cre-loxP 单质粒合成难题，开展 TAx9 相关研究，成功创建 F0 代转基因动物，意义重大。 开启基因编辑新征程：TAx9 技术的诞生 在生命科学的奇妙世界里，基因编辑技术宛如一把神奇的 “剪刀”，帮助科学家们探索生命的奥秘。其中，Cre-loxP 系统 ...

The most straightforward way to examine the pattern of Cre-mediated recombination in the various tissues of the mouse is Southern blot analysis distinguishing wild-type, loxP-flanked and ...

Related Links: Jackson Laboratory / Mutant Mouse Regional Resource Center (MMRRC ... On binding tamoxifen, the complex is translocated to the nucleus where cre activates recombination of loxP-flanked ...

The offspring are heterozygous for the floxed target gene (C) and breed with the homozygous floxed mouse (B). The resulting experimental mouse is hemizygous for Cre and homozygous for loxP (D). This ...

The Yong Xu lab is interested in novel neural circuits, neurotransmitters and intra-neuronal signals that are critical for coordinated control of metabolism and behavior. We generate unique mouse ...

On adding Cre recombinase, the enzyme recognized the loxP sites and excised the STOP codon. Thus, active GFP expression served as an indicator of successful Cre delivery into the cells. But the true ...

All our multiexpression systems (currently MultiBac, MultiMam and MultiColi) rely on in vitro Cre-loxP recombination of Acceptor and Donor plasmids to assemble multiprotein expression vectors. A ...