In addition, loxP-flanked target genes can differ dramatically ... On the other hand, most mice generated via knock-in of Cre-encoding sequences into endogenous loci are heterozygous knockouts ...

CRISPR/Cas9-initiated HDR in mouse embryos will be used to generate founder animals harboring inserted sequence (point mutations, epitope tags, loxP sites) or specific interval deletion alleles. At ...

Now imagine that one can use genetic manipulation to create, at will, singly-labeled neurons in intact brain tissue or in vivo, and moreover, knock out ... as FLP/FRT or Cre/LoxP, not only can ...