Figure 1: Large-scale strategy for mutagenesis of the mouse genome: a conditional knockout vector is created for each gene and introduced into mouse embryonic stem (ES) cells. The mechanisms ...

Conditional gene targeting in mice allows the introduction of targeted mutations in a cell type–specific and/or inducible way. This is most commonly achieved by flanking a target gene segment ...

Both gene knockout and knock-in models are powerful tools widely used in biomedical research to gain important insights into ...