1| Combine the following components of the master mix on ice and ... Note, the pH is 9.2 (the optimal for long PCR) without adjustment; some vectors may require pH 7.2. 10-40 dNTP mix: 10 mM ...

PCR, or Polymerase Chain Reaction, is a revolutionary molecular biology technique that allows for the rapid amplification of specific DNA sequences. It is an essential tool in various fields, ...

Figure 1: Amplification of complex gene libraries by conventional PCR and emulsion PCR ... should be performed at 25 °C using 25 °C components for the main protocol and on ice using ice-cold ...