Identity of the active species. A quality chemical probe has sufficient chemical and physical property data to permit utilization in in vitro and cell-based assays with interpretations of results ...
The simplest version of a drop-off assay includes two TaqMan™ probes targeting the same amplicon: a drop-off probe that spans the mutation hotspot but is uniquely complementary to the wild-type ...
predesigned assays, and separate custom primers and probes. Such assays are suitable for all qPCR applications, such as microbial detection, copy number analysis, gene expression analysis ...